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Flow Cytometry Analysis of Cell-miRNA and EV-miRNA Signal in EVs Isolated from Calu6 Cells Transfected with Fluorophore-Conjugated EV-miRNA
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Biologia
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JoVE Journal Biologia
Flow Cytometry Analysis of Cell-miRNA and EV-miRNA Signal in EVs Isolated from Calu6 Cells Transfected with Fluorophore-Conjugated EV-miRNA
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Flow Cytometry Analysis of Cell-miRNA and EV-miRNA Signal in EVs Isolated from Calu6 Cells Transfected with Fluorophore-Conjugated EV-miRNA

A Flow Cytometry Protocol for Understanding Export Mechanisms

DOI:
10.3791/201250-v

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03:01 min

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October 06, 2023

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1Department of Biological Sciences,Purdue University, 2Purdue Institute for Cancer Research,Purdue University

Tags

Flow CytometryExtracellular VesiclesEV-miRNACell-miRNAFluorophore-conjugated MiRNACalu6 CellsNano-flow CytometryPerformance BeadsInstrument CalibrationGatingSample PreparationData Analysis

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