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Fabrication Followed by 3D Encapsulation of Tumor Spheroids in PEG Hydrogels

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Immunofluorescence Fixation, Staining, and Clearing of Encapsulated Spheroids for Confocal Microscopy

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JoVE Journal Bioingegneria

Immunofluorescence Fixation, Staining, and Clearing of Encapsulated Spheroids for Confocal Microscopy

Immunofluorescence Fixation, Staining, and Clearing of Encapsulated Spheroids for Confocal Microscopy

Fabrication Followed by 3D Encapsulation of Tumor Spheroids in PEG Hydrogels

DOI:

10.3791/201261-v

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03:35 min

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September 22, 2023

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Joseph Bruns, Shabnam Nejat, Allison Faber, Silviya P. Zustiak

¹Biomedical Engineering Department,Saint Louis University

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Keywords: Immunofluorescence Fixation Staining Clearing Encapsulated Spheroids Confocal Microscopy Hydrogel PBS Fixative Primary Antibodies Nestin SOX2 Formamide Z-stack Maximum Projection DAPI Stem Cell Marker

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