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Development of a Click-Based Assay for Screening HAT1 Enzyme Activity
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Click Assay for the Measurement of Histone Acetyltransferase 1 Acetylation
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Biochimica
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JoVE Journal Biochimica
Click Assay for the Measurement of Histone Acetyltransferase 1 Acetylation

Click Assay for the Measurement of Histone Acetyltransferase 1 Acetylation

Development of a Click-Based Assay for Screening HAT1 Enzyme Activity

DOI:
10.3791/201342-v

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04:18 min

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January 26, 2024

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, , ,
1Departments of Internal Medicine and the Green Center for Reproductive Biology Sciences,University of Texas Southwestern Medical Center, 2Department of Pharmaceutical Sciences, Eugene Applebaum College of Pharmacy and Health Sciences,Wayne State University

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Keywords: Click AssayHistone Acetyltransferase 1 AcetylationBiotinylated Histone H4 PeptideAcetylation Reactions4-Penten Oil Co-enzyme ABSA Pre-blocked Black NeutrAvidin-coated 96-well PlatesTHPTA Copper MixtureSodium AscorbateBiotin AzideStreptavidin Horseradish PeroxidaseAmplex Red Detection ReagentsHydrogen PeroxideSodium Phosphate

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