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Protein Interaction Analysis in HEK-293 Cells Using Co-Immunoprecipitation

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Eluting Epitope Tag Precipitated Proteins and Immunoblotting for Protein-Protein Interaction Studies

JoVE Journal
Biologia

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JoVE Journal Biologia

Eluting Epitope Tag Precipitated Proteins and Immunoblotting for Protein-Protein Interaction Studies

Eluting Epitope Tag Precipitated Proteins and Immunoblotting for Protein-Protein Interaction Studies

Protein Interaction Analysis in HEK-293 Cells Using Co-Immunoprecipitation

DOI:

10.3791/201505-v

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02:58 min

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January 05, 2024

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Hiroko Shinjo, Gaku Nagano, Shogo Ishii, Natsumi Himeno, Yutaro Yamamoto, Junji Sagawa, Ryuta Baba, Gentaro Egusa, Noboru Hattori, Haruya Ohno

¹Department of Molecular and Internal Medicine, Graduate School of Biomedical & Health Sciences,Hiroshima University

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Keywords: Epitope Tag Protein Precipitation Immunoblotting Protein-protein Interaction Centrifugation Protein Lysis Buffer Protease Inhibitor Sample Buffer SDS-PAGE PVDF Membrane Primary Antibody DYKDDDDK-PRDM16 HA-EHMT1 HEK 293 Cells

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