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大肠杆菌细胞的转化用于评估 Hsp70 伴侣活性的互补测定

JoVE Journal
Biochimica

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JoVE Journal Biochimica

Transformation of E. coli Cells for a Complementation Assay to Assess Hsp70 Chaperone Activity

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大肠杆菌细胞的转化用于评估 Hsp70 伴侣活性的互补测定

DOI:

10.3791/201542-v

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03:31 min

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March 08, 2024

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Hloniphani Rachel Ncube, Unarine Dali, Tendamudzimu Harmfree Dongola, Addmore Shonhai

¹Department of Biochemistry & Microbiology,University of Venda

Tags

E. Coli DnaK756 Complementation Assay Hsp70 Chaperone Activity Heat Shock Plasmid DNA Transformation Agar Plates Antibiotic Selection Serial Dilutions Permissive And Non-permissive Growth Temperatures Recombinant Protein Expression KPf KPf-V436F

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