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Inverted Fluorescence Microscopy and Data Analysis to Assess Drug Effects on cAMP in HASM Cells

JoVE Journal
Biochimica

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JoVE Journal Biochimica

Inverted Fluorescence Microscopy and Data Analysis to Assess Drug Effects on cAMP in HASM Cells

Inverted Fluorescence Microscopy and Data Analysis to Assess Drug Effects on cAMP in HASM Cells

DOI:

10.3791/201672-v

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02:33 min

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March 22, 2024

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Isabella Cattani-Cavalieri, Jordyn Margolis, Camelia Anicolaesei, Francisco J. Nuñez, Rennolds S. Ostrom

¹Department of Biomedical and Pharmaceutical Sciences,Chapman University School of Pharmacy

Tags

CAMP Biosensor CADDis Epac2 Fluorescent Protein CAMP Dynamics Real-time Measurement HEK-293 Cells HASM Cells 96-well Plate Fluorescent Microscopy High-throughput Screening CAMP Signaling

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