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Capitoli
- 00:04Microfabrication of channels
- 00:16Mask
- 00:55Cleaning and baking the wafer
- 02:11Coating the wafer
- 04:13Soft baking the wafer
- 04:47UV exposure
- 06:14Developing the wafer
- 08:19Application of PDMS to produce channels
- 12:44Experiment 1: Investigating the chemotactic response of marine micr
- 13:44Adding organisms and substrates to glass syringes
- 14:39Assembling the microfluidics system on the microscope stage
- 15:53Observation under microscope
- 16:37Removing bubbles from channel
- 17:59Visualization of nutrient gradient and microbes
- 18:42Measurement of chemotaxis
- 19:47Experiemnt 2: Investigating the effects of shear on marine bacteria
Die Herstellung von mikrofluidischen Kanälen und deren Umsetzung in Experimenten zur Untersuchung der chemotaktischen Jagdverhalten von marinen Mikroben innerhalb eines lückenhaft Nährstoff seascape und das Schwimmverhalten der Bakterien innerhalb Scherströmung beschrieben sind.
Tags
Chemotactic ResponseMarine Micro-organismsMicro-scale Nutrient LayersPlanktonic MicrobesResource PatchesOceanic TrophodynamicsBiogeochemical FluxPhysical ForcesMolecular DiffusionTurbulent ShearNutrient PatchesBacteria LocationMethodological LimitationsMicrobial BehaviourPatchy HabitatsSmall-scale Flow ConditionsSoft Lithographic Fabrication TechniquesMicrofluidic DevicesMicroscale Nutrient PatchesOceanic ProcessesMicroscale VorticesShear RatesMicrobial SwimmingChemotactic BehaviourHeterogeneous SeascapeEpifluorescence MicroscopyPhase Contrast Microscopy
