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Capitoli
- 00:04Microfabrication of channels
- 00:16Mask
- 00:55Cleaning and baking the wafer
- 02:11Coating the wafer
- 04:13Soft baking the wafer
- 04:47UV exposure
- 06:14Developing the wafer
- 08:19Application of PDMS to produce channels
- 12:44Experiment 1: Investigating the chemotactic response of marine micr
- 13:44Adding organisms and substrates to glass syringes
- 14:39Assembling the microfluidics system on the microscope stage
- 15:53Observation under microscope
- 16:37Removing bubbles from channel
- 17:59Visualization of nutrient gradient and microbes
- 18:42Measurement of chemotaxis
- 19:47Experiemnt 2: Investigating the effects of shear on marine bacteria
La fabricación de los canales de microfluidos y su aplicación en los experimentos para estudiar el comportamiento quimiotáctico de alimentación de los microbios marinos dentro de un paisaje marino irregular de nutrientes y el comportamiento de la natación de las bacterias dentro del flujo de corte se describen.
Tags
Chemotactic ResponseMarine Micro-organismsMicro-scale Nutrient LayersPlanktonic MicrobesResource PatchesOceanic TrophodynamicsBiogeochemical FluxPhysical ForcesMolecular DiffusionTurbulent ShearNutrient PatchesBacteria LocationMethodological LimitationsMicrobial BehaviourPatchy HabitatsSmall-scale Flow ConditionsSoft Lithographic Fabrication TechniquesMicrofluidic DevicesMicroscale Nutrient PatchesOceanic ProcessesMicroscale VorticesShear RatesMicrobial SwimmingChemotactic BehaviourHeterogeneous SeascapeEpifluorescence MicroscopyPhase Contrast Microscopy
