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Mouse Embryonic Lung Culture, A System to Evaluate the Molecular Mechanisms of Branching
JoVE Journal
Biologia
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JoVE Journal Biologia
Mouse Embryonic Lung Culture, A System to Evaluate the Molecular Mechanisms of Branching

Mouse Embryonic Lung Culture, A System to Evaluate the Molecular Mechanisms of Branching

DOI:
10.3791/2035-v

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07:32 min

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June 30, 2010

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, ,
1Saban Research Institute,Childrens Hospital Los Angeles

Capitoli

  • 00:00Titolo
  • 00:39Introduction
  • 01:22Embryonic Lung Isolation
  • 05:22Embryonic Lung Culture
  • 06:21Representative Lung Cultures
  • 07:07Conclusion

Summary

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Early embryonic lung organ culture is a very useful system to study epithelial-mesenchymal interactions. Both epithelial and mesenchymal morphogenesis proceeds under specific conditions that can be readily manipulated in this system.

Tags

Mouse Embryonic Lung CultureMolecular MechanismsBranchingLung Primordial SpecificationBranching MorphogenesisPulmonary Cell LineagesLung EndodermSurrounding MesenchymeMesotheliumInductive SignalsEpithelial-mesenchymal-mesothelial InteractionsEmbryonic Lung Organ CultureEpithelial-mesenchymal InteractionsSerumless Culture MediaGain Of FunctionLoss Of FunctionExpressed ProteinsRecombinant Viral VectorsTransgenic Mouse StrainsAntisense RNARNA Interference Gene Knockdown

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