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在录制多细胞行为黏细菌生物膜，使用定时Microcinematography
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Biologia
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JoVE Journal Biologia
Recording Multicellular Behavior in Myxococcus xanthus Biofilms using Time-lapse Microcinematography
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在录制多细胞行为黏细菌生物膜，使用定时Microcinematography

DOI:
10.3791/2038-v

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10:59 min

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August 06, 2010

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1Department of Environmental Health Sciences,University of South Carolina (USC), 2Department of Biology,Syracuse University

Capitoli

  • 00:04Titolo
  • 00:49Introduction
  • 01:11Preparing Bacteria and Nutritive Disk
  • 02:59Setting Up Plate Complexes
  • 06:19Plating Cells and Assembling Assay
  • 07:56Preparing the Movie
  • 08:45Time-Lapse Microcinematography
  • 10:35Conclusion

Summary

Traduzione automatica

Traduzione automatica

研究<em>黏细菌</em>群的行为，我们设计了一个时间推移microcinematography协议，可用于不同的检测修改。它采用的是标准的显微镜适应增长的条件下，使用价格低廉，可重复使用的硅胶垫片，并产生可重复的结果。我们已经用这种方法来量化的多细胞的趋化。

Tags

Multicellular BehaviorMyxococcus XanthusBiofilmsTime-lapse MicrocinematographySwarmδ-proteobacteriumCollective BehaviorCoordinationComplex PatternsEnvironmental CuesSelf-organizingEmergent PatternsChemotaxisNutrient GradientTime-lapse Microcinematography Tracking AssayPlate ComplexMicroscopesTime-lapse VideosQuantifiable DataAnalysis/storage ComputerM. Xanthus Community

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