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Capitoli
- 00:05Titolo
- 01:02Introduction
- 01:32Dechorionating Medaka Eggs
- 04:22Cell Transplantation in Medaka Embryos
- 06:05Mounting Dechorionated Embryos
- 07:35Images of Post-Transplantation Embryos
- 08:31Conclusion
Due to the hard chorion and soft embryos, manipulation of medaka embryos is more involved than in zebrafish. This video shows step-by-step procedures for how to manipulate medaka embryos, including dechorionation, mounting in agarose for imaging and cell transplantation for the production of chimeras. These procedures are essential to use medaka and zebrafish in a laboratory to take full advantage of their complementary features for the genetic dissection of vertebrate genome functions.
