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How to Culture, Record and Stimulate Neuronal Networks on Micro-electrode Arrays (MEAs)
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Biologia
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JoVE Journal Biologia
How to Culture, Record and Stimulate Neuronal Networks on Micro-electrode Arrays (MEAs)

How to Culture, Record and Stimulate Neuronal Networks on Micro-electrode Arrays (MEAs)

DOI:
10.3791/2056-v

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09:27 min

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May 30, 2010

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, ,
1Department of Neurology,Emory University School of Medicine, 2Coulter Department of Biomedical Engineering, Laboratory for Neuroengineering, Georgia Institute of Technology and Emory,University School of Medicine, 3Emory University School of Medicine

Capitoli

  • 00:00Titolo
  • 00:51Introduction
  • 01:30Culturing Cortical Neurons on a Micro-Electrode Array (MEA)
  • 05:03Recording From Neuronal Networks on the MEA
  • 07:54Representative Recordings from Cultures On MEAs
  • 08:44Conclusion

Summary

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This protocol provides the necessary information for setting up, caring for, recording from and electrically stimulating cultures on MEAs. In vitro networks provide a means for asking physiologically relevant questions at the network and cellular levels leading to a better understanding of brain function and dysfunction.

Tags

CultureRecordStimulateNeuronal NetworksMicro-electrode Arrays (MEAs)NeuroscientistsUnderstandingDiseasesNeuropathological ObservationFluorescent MicroscopyGenetic LabelingIntracellular RecordingDissociated NeuronsSlice PreparationsGrowing Dissociated Neuronal CulturesAdvantagesSimplified ModelNetwork Activity ManipulationElectrical Stimulation SequencesMultiple ElectrodesObservable AreasMonolayer CellsMorphology ChangesImaging TechniquesLong-term Survival In Vitro

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