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1Epigenomic Medicine, Baker IDI Heart and Diabetes Institute,The Alfred Medical Research and Education Precinct, 2Epigenetics in Human Health and Disease, Baker IDI Heart and Diabetes Institute,The Alfred Medical Research and Education Precinct, 3Department of Pathology,The University of Melbourne, 4Department of Allergy and Immunology, Murdoch Children’s Research Institute,Royal Children’s Hospital, 5Department of Pediatrics,The University of Melbourne
Capitoli
- 00:00Titolo
- 00:05Introduction
- 01:42Tissue Preparation
- 02:29Immunofluorescence Staining
- 05:19Microscopy
- 07:02Analysis
- 08:24Conclusion
Kwantificering van DNA dubbelstrengs breuken aan de hand van γH2AX foci is uitgegroeid tot een instrument van onschatbare waarde, met name in de biologie straling, voor de evaluatie van weefsel stralingsgevoeligheid en de effecten van de straling wijzigen van verbindingen. Hier laten we zien het gebruik van een immunofluorescentie test voor kwantificatie van γH2AX foci in weefselmonsters.
Tags
QuantitationγH2AX FociDNA Double-strand BreaksEndogenous ProcessesExogenous FactorsIonizing RadiationRadiomimetic CompoundsH2A Histone VariantSerine-139 ResiduePhosphorylationPI3K Family Of ProteinsATM KinaseATR KinaseNuclear γH2AX FociImmunofluorescence MicroscopyMarker Specificity And SensitivityBiomedical ResearchRadiation BiologyNuclear MedicineCell Culture SystemsIonizing Radiation-induced DSBsCellular RadiosensitivityRadiation-modifying CompoundsDSB-inducing Compounds
