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1Epigenomic Medicine, Baker IDI Heart and Diabetes Institute,The Alfred Medical Research and Education Precinct, 2Epigenetics in Human Health and Disease, Baker IDI Heart and Diabetes Institute,The Alfred Medical Research and Education Precinct, 3Department of Pathology,The University of Melbourne, 4Department of Allergy and Immunology, Murdoch Children’s Research Institute,Royal Children’s Hospital, 5Department of Pediatrics,The University of Melbourne
Capitoli
- 00:00Titolo
- 00:05Introduction
- 01:42Tissue Preparation
- 02:29Immunofluorescence Staining
- 05:19Microscopy
- 07:02Analysis
- 08:24Conclusion
La quantification de l'ADN double-brin de brise sur la base de γH2AX foyers est devenu un outil précieux, en particulier dans la radiobiologie, pour l'évaluation de la radiosensibilité des tissus et des effets des rayonnements composés de modification. Ici nous démontrons l'utilisation d'un test d'immunofluorescence pour la quantification des γH2AX foyers dans des échantillons de tissus.
Tags
QuantitationγH2AX FociDNA Double-strand BreaksEndogenous ProcessesExogenous FactorsIonizing RadiationRadiomimetic CompoundsH2A Histone VariantSerine-139 ResiduePhosphorylationPI3K Family Of ProteinsATM KinaseATR KinaseNuclear γH2AX FociImmunofluorescence MicroscopyMarker Specificity And SensitivityBiomedical ResearchRadiation BiologyNuclear MedicineCell Culture SystemsIonizing Radiation-induced DSBsCellular RadiosensitivityRadiation-modifying CompoundsDSB-inducing Compounds
