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1Epigenomic Medicine, Baker IDI Heart and Diabetes Institute,The Alfred Medical Research and Education Precinct, 2Epigenetics in Human Health and Disease, Baker IDI Heart and Diabetes Institute,The Alfred Medical Research and Education Precinct, 3Department of Pathology,The University of Melbourne, 4Department of Allergy and Immunology, Murdoch Children’s Research Institute,Royal Children’s Hospital, 5Department of Pediatrics,The University of Melbourne
Capitoli
- 00:00Titolo
- 00:05Introduction
- 01:42Tissue Preparation
- 02:29Immunofluorescence Staining
- 05:19Microscopy
- 07:02Analysis
- 08:24Conclusion
Die Quantifizierung der DNA-Doppelstrangbrüche auf der Grundlage γH2AX Brennpunkten hat sich zu einem wertvollen Werkzeug, vor allem in der Strahlenbiologie, für die Auswertung von Gewebe Strahlenempfindlichkeit und die Auswirkungen der Strahlung modifizierende Verbindungen. Hier zeigen wir die Verwendung eines Immunfluoreszenztest zur Quantifizierung von γH2AX Brennpunkte in Gewebeproben.
Tags
QuantitationγH2AX FociDNA Double-strand BreaksEndogenous ProcessesExogenous FactorsIonizing RadiationRadiomimetic CompoundsH2A Histone VariantSerine-139 ResiduePhosphorylationPI3K Family Of ProteinsATM KinaseATR KinaseNuclear γH2AX FociImmunofluorescence MicroscopyMarker Specificity And SensitivityBiomedical ResearchRadiation BiologyNuclear MedicineCell Culture SystemsIonizing Radiation-induced DSBsCellular RadiosensitivityRadiation-modifying CompoundsDSB-inducing Compounds
