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在原位议定书“蝴蝶蛹使用Riboprobes翼
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Biologia
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JoVE Journal Biologia
In situ Protocol for Butterfly Pupal Wings Using Riboprobes
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在原位议定书“蝴蝶蛹使用Riboprobes翼

DOI:
10.3791/208-v

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06:19 min

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May 28, 2007

•

,
1Department of Biological Sciences,SUNY-University at Buffalo, 2Dept. Ecology and Evolutionary Biology,Yale University

Capitoli

  • 00:09Collecting pre-pupae
  • 00:36Dissecting and fixing wings
  • 01:08Pre-hybridization
  • 02:27Making hybridization buffer
  • 03:09Hybridization
  • 03:59Applying antibody
  • 04:31Detection and development
  • 05:35Mounting
  • 05:59Result: patterns of Engrailed

Summary

Traduzione automatica

Traduzione automatica

为了检验在Bicyclus anynana蛹右翼组织的基因表达，我们目前在使用riboprobes原位杂交优化协议。我们还提供指引，为进一步优化本议定书在其他鳞翅目物种蛹翅膀。

Tags

In Situ ProtocolButterfly Pupal WingsRiboprobesIn Situ HybridizationsGene ExpressionTranscriptionBiologia dello sviluppoSpatial PatternsTemporal PatternsAntibodiesProtein ProductsLarval Wing DiscsFragile Pupal Wings

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