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Capitoli
- 00:00Titolo
- 00:46Introduction
- 01:21Overview of the DR-CARS Microscopy System
- 05:22The Generation of CARS and Doubly-resonant CARS Signals
- 07:02Sample Preparation
- 11:16Imaging Results
- 12:20Conclusion
A combination of three single wavelength short-pulsed lasers is used to generate coherent anti-Stokes Raman scattering (CARS) and doubly-resonant CARS (DR-CARS). The difference between these signals provides enhanced sensitivity for otherwise difficult to detect coherent Raman signals, enabling imaging of weak Raman scatterers.
Tags
Differential ImagingBiological StructuresDoubly-resonantCoherent Anti-stokes Raman Scattering (CARS)Coherent Raman ImagingLabel-free Optical ImagingMolecular SpecificitySensitivityFluorescenceWeak ConcentrationsRaman-active MoleculesSignal AmplificationPulsed LasersCoherent Raman Scattering SignalsCoherent Anti-stokes Raman Scattering (CARS)3-color CARSFour-wave Mixing (FWM)Doubly-resonant CARS (DR-CARS)Constructive InterferenceTuning LasersCH Stretch VibrationCD Stretching Vibration
