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髄芽腫の幹細胞の単離、濃縮、およびメンテナンス
JoVE Journal
Neuroscienze
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JoVE Journal Neuroscienze
Isolation, Enrichment, and Maintenance of Medulloblastoma Stem Cells
DOI:

06:32 min

September 01, 2010

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Capitoli

  • 00:00Titolo
  • 00:59Introduction
  • 01:20Micro-dissection of Tumor-bearing Cerebellum and Dissociation of Tumor Tissue
  • 03:34Enrichment, Maintenance, and Expansion of Medulloblastoma Tumor Cells by Serial Passages
  • 04:26Immunofluorescent Staining of Cultured Medulloblastoma Cells
  • 04:53Morphology, Lineage Differentiation, and Expression of Stem Cells Markers
  • 06:17Conclusion

Summary

Traduzione automatica

このプロトコルは、分離、濃縮、および異所性ソニックヘッジホッグ経路の活性をもつ変異マウス由来の髄芽腫の腫瘍幹細胞の維持を説明します。

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