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Axoplasm Isolation from Rat Sciatic Nerve
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JoVE Journal Neuroscienze
Axoplasm Isolation from Rat Sciatic Nerve

Axoplasm Isolation from Rat Sciatic Nerve

DOI:
10.3791/2087-v

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05:29 min

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September 24, 2010

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, ,
1Department of Biological Chemistry,Weizmann Institute of Science

Capitoli

  • 00:00Titolo
  • 00:50Introduction
  • 01:38Dissection of Sciatic Nerves
  • 03:16Incubation, Washing, and Elution of Axoplasm
  • 04:41Evaluation of Axoplasm Purity
  • 05:06Conclusion

Summary

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We demonstrate a protocol for axoplasm isolation from adult rat sciatic nerve based on dissection of nerve fascicles and incubation in hypotonic medium to release myelin and lyse non-axonal structures, followed by extraction of the remaining axon-enriched material.

Tags

Axoplasm IsolationRat Sciatic NervePeripheral NerveBiochemical StudiesProtocolDissectionHypotonic MediumMyelinNon-axonal StructuresAxon-enriched MaterialProteomic CharacterizationBiochemical CharacterizationAxonal Components

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