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An Assay for Measuring the Activity of Escherichia coli Inducible Lysine Decarboxyase
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Biologia
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JoVE Journal Biologia
An Assay for Measuring the Activity of Escherichia coli Inducible Lysine Decarboxyase

An Assay for Measuring the Activity of Escherichia coli Inducible Lysine Decarboxyase

DOI:
10.3791/2094-v

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08:08 min

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December 19, 2010

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,
1Department of Biochemistry,University of Toronto

Capitoli

  • 00:05Titolo
  • 01:10Introduction
  • 01:39Enzyme Assay Preparation
  • 02:51Lysine Decarboxylase (Ldcl) Enzyme Assay
  • 04:17Trinitrobenzene Sulfonic Acid (TNBS) Reaction and Color Development
  • 06:42Ldcl Assay Representative Results
  • 07:31Conclusion

Summary

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The activity of the inducible lysine decarboxylase is monitored by reacting the substrate L-lysine and the product cadaverine with 2,4,6-trinitrobenzensulfonic acid to form adducts that have differential solubility in toluene.

Tags

AssayMeasuringActivityEscherichia ColiInducible Lysine DecarboxyasePHAcidic PH SurvivalAmino Acid DecarboxylasesGlutamic Acid DecarboxylasesArginine DecarboxylaseLysine DecarboxylaseOrnithine DecarboxylasePyridoxal-5′-phosphateCo-factorInner-membrane Substrate-product AntiportersLysine-cadaverine AntiporterCadBX-ray Crystal StructureStringent Response Regulator Guanosine 5′-diphosphate 3′-diphosphatePpGppStringent Response

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