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In vivo Micro-circulation Measurement in Skeletal Muscle by Intra-vital Microscopy
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JoVE Journal Biologia
In vivo Micro-circulation Measurement in Skeletal Muscle by Intra-vital Microscopy

In vivo Micro-circulation Measurement in Skeletal Muscle by Intra-vital Microscopy

DOI:
10.3791/210-v

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07:31 min

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May 28, 2007

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, , , ,
1Department of Anesthesiology and Critical Care, Shriners Hospital for Children,Massachusetts General Hospital, and Harvard Medical School, 2Department of Geriatric Medicine, Graduate School of Medicine,The University of Tokyo

Capitoli

  • 00:28Introduction
  • 02:09Mouse preparation
  • 03:08Red blood cell staining and injection
  • 04:49Discussion

Summary

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A new versatile method for observation of microcirculation is presented. It is considered suitable for long-term observation, and for combination with pharmacophysiological or molecular biological interventions.

Tags

In Vivo MicrocirculationSkeletal MuscleIntra-vital MicroscopyBlood Flow VelocityRed Blood Cell FluxMacroscopic ParametersImaging ModalitiesRBC VelocityRBC FluxPlasma Flow VolumeSpatial And Temporal HeterogeneityFlow RegulationMicrocirculatory RegulationIntravital MicroscopyJeff Lichtman’s MethodMouse Sternomastoid MusclesPKH26L-fluorescently Labeled RBCsWide-field MicroscopeMuscle ContractionElectrical StimulationRBC VelocityCapillary Density

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