Rivista
/
Biologia
/
Genome-wide Analysis using ChIP to Identify Isoform-specific Gene Targets
JoVE Journal
Biologia
This content is Free Access.
JoVE Journal Biologia
Genome-wide Analysis using ChIP to Identify Isoform-specific Gene Targets

Genome-wide Analysis using ChIP to Identify Isoform-specific Gene Targets

DOI:
10.3791/2101-v

•

11:19 min

•

July 07, 2010

•

, , , , , ,
1Department of Biochemistry and Molecular Genetics,University of Illinois Chicago – UIC, 2Research Unit on Biomedical Informatics,Universitat Pompeu Fabra, 3Genome Technology Core,Whitehead Institute for Biomedical Research

Capitoli

  • 00:00Titolo
  • 00:53Introduction
  • 01:33Cell Cross-linking
  • 02:39Cell Sonication
  • 04:03Chromatin Immunoprecipitation (ChIP)
  • 07:06Amplification of Genomic DNA
  • 08:27Gel Purification of Amplified Products
  • 09:46Representative Results for Genome-wide Analysis Using ChIP
  • 10:53Conclusion

Summary

Traduzione automatica

Traduzione automatica

Here we are presenting a chromatin immunoprecipitation (ChIP) procedure for genome-wide location analysis of protein isoforms that differ in a histone-binding domain. We are applying it to ChIP-Seq analysis to identify the targets of the KDM5A/JARID1A/RBP2 histone demethylase.

Tags

Genome-wide AnalysisChIPIsoform-specific Gene TargetsTranscriptional FactorsEpigenetic FactorsProtein DomainsPlant Homeodomain (PHD)Histone ModificationsRBP2Oncogenic FusionHuman LeukemiaTrimethylated Lysine 4Histone H3 (H3K4me3)RBP2 IsoformDifferentiationU937 CellsGenomic RegionsHistone Demethylase ActivityTranscriptional Activity

Article

Embed

AGGIUNGI ALLA PLAYLIST

Usage Statistics

Video correlati

check_url/it/2101?article_type=v

Bacterial Gene Expression Analysis Using Microarrays
check_url/it/2101?article_type=v

Genome-wide Analysis of Aminoacylation (Charging) Levels of tRNA Using Microarrays
check_url/it/2101?article_type=v

Genome-wide Screen for miRNA Targets Using the MISSION Target ID Library
check_url/it/2101?article_type=v

Quantitative and Automated High-throughput Genome-wide RNAi Screens in C. elegans
check_url/it/2101?article_type=v

Isolation of Ribosome Bound Nascent Polypeptides in vitro to Identify Translational Pause Sites Along mRNA
check_url/it/2101?article_type=v

A Novel Bayesian Change-point Algorithm for Genome-wide Analysis of Diverse ChIPseq Data Types
check_url/it/2101?article_type=v

Genome-wide Gene Deletions in Streptococcus sanguinis by High Throughput PCR
check_url/it/2101?article_type=v

Polysome Fractionation and Analysis of Mammalian Translatomes on a Genome-wide Scale
check_url/it/2101?article_type=v

DNA-affinity-purified Chip (DAP-chip) Method to Determine Gene Targets for Bacterial Two component Regulatory Systems
check_url/it/2101?article_type=v

Genome-wide Mapping of Drug-DNA Interactions in Cells with COSMIC (Crosslinking of Small Molecules to Isolate Chromatin)

Read Article