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The Organotypic Hippocampal Slice Culture Model for Examining Neuronal Injury
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Neuroscienze
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JoVE Journal Neuroscienze
The Organotypic Hippocampal Slice Culture Model for Examining Neuronal Injury

The Organotypic Hippocampal Slice Culture Model for Examining Neuronal Injury

DOI:
10.3791/2106-v

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06:33 min

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October 28, 2010

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1Department of Neurology and Neurological Sciences,Stanford University School of Medicine

Capitoli

  • 00:00Titolo
  • 01:39Preparing Slice Cultures
  • 03:51Inducing and Quantifying Hippocampal Neuronal Injury in Slice Culture
  • 05:43Representative Images
  • 06:13Conclusion

Summary

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The organoptypic hippocampal slice culture model is an in vitro model used to examine neuronal injury in a variety of paradigms. In this article, we describe the methods for generating slice cultures and quantifying neuronal injury.

Tags

Organotypic Hippocampal Slice CultureNeuronal InjuryIn Vitro MethodHippocampusNeuronal ArchitectureCompositionOrganotypic Culture SystemAstrocytic EffectsMicroglial EffectsEx Vivo PreparationBlood Flow EffectsPeripheral Inflammatory CellsExcitotoxic InjuryHypoxic InjuryPyramidal NeuronsInflammatory ResponseMethods For Generating Hippocampal Slice CulturesToxic StimuliAssaying And Quantifying Neuronal Death

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