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Generation of RNA/DNA Hybrids in Genomic DNA by Transformation using RNA-containing Oligonucleotides
JoVE Journal
Biologia
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JoVE Journal Biologia
Generation of RNA/DNA Hybrids in Genomic DNA by Transformation using RNA-containing Oligonucleotides

Generation of RNA/DNA Hybrids in Genomic DNA by Transformation using RNA-containing Oligonucleotides

DOI:
10.3791/2152-v

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16:42 min

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November 24, 2010

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,
1School of Biology,Georgia Institute of Technology

Capitoli

  • 00:05Titolo
  • 01:20Introduction
  • 01:53Preparation of the RNA-containing Oligo and the Yeast Cells for Transformation
  • 04:40Transformation of Yeast Cells Using the RNA-Containing Oligo
  • 07:47Analysis of Gene Correction by the RNA-Containing Oligo
  • 11:10Alkali Treatment of the RNA-Containing Oligo
  • 12:20Representative Results for Transformation Using RNA-Containing Oligonucleotides
  • 16:10Conclusion

Summary

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Traduzione automatica

This work shows how to form an RNA/DNA hybrid at the chromosomal level and reveal transfer of genetic information from RNA to genomic DNA in yeast cells.

Tags

RNA/DNA HybridsGenomic DNATransformationRNA-containing OligonucleotidesSynthetic Short Nucleic Acid PolymersOligonucleotidesMolecular Biology ToolsBase And Sugar ModificationsNucleic Acid AlterationsDNA DamageMechanisms Of RepairThermo Scientific Dharmacon RNA-containing OligosIn Vivo StudyChromosomal RNA/DNA HybridsRibonucleotides Embedded Into DNAGenetic Integrity Of CellsRibonucleotide IncorporationEukaryotic Model System Yeast/Saccharomyces Cerevisiae

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