È necessario avere un abbonamento a JoVE per visualizzare questo. Accedi o inizia la tua prova gratuita.
Capitoli
- 00:05Titolo
- 01:13Introduction
- 01:42Isolation of Heart Valve Leaflets
- 03:03Isolation of Endothelial Layer
- 05:22Isolation of Side Specific Endothelial Layer
- 08:03Isolation of Interstitial Cells
- 09:07Isolation of Valvular Endothelial Cells Representative Images
- 10:30Conclusion
Tags
IsolationValvular Endothelial CellsHeart ValvesUnidirectional Blood FlowCardiovascular SystemMechanical StressesValvular Endothelial CellsInterstitial CellsRepair And RemodelIn Vitro ExperimentationIsolation And CultureDirect IsolationSide Specific IsolationCultureVerification Of Pure PopulationsEnzymatic DigestionGentle Swab Scraping TechniqueSurface CellsCentrifuged Into A PelletResuspended And PlatedCollagen I MatrixVEC PhenotypeContact Inhibited GrowthEndothelial Specific Markers (PECAM1/CD31)Von Willebrand Factor (vWF)Alpha-smooth Muscle Actin (α-SMA)Functional Characteristics
