Rivista
/
Immunologia e infezioni
/
Transnuclear Mice with Pre-defined T Cell Receptor Specificities Against Toxoplasma gondii Obtained Via SCNT
JoVE Journal
Immunologia e infezioni
This content is Free Access.
JoVE Journal Immunologia e infezioni
Transnuclear Mice with Pre-defined T Cell Receptor Specificities Against Toxoplasma gondii Obtained Via SCNT

Transnuclear Mice with Pre-defined T Cell Receptor Specificities Against Toxoplasma gondii Obtained Via SCNT

DOI:
10.3791/2168-v

•

13:36 min

•

September 30, 2010

•

, , , , ,
1Whitehead Institute for Biomedical Research, 2Departments of Microbiology and Biological Sciences,National University of Singapore, 3Department of Biology,Massachusetts Institute of Technology

Capitoli

  • 00:00Titolo
  • 00:15Introduction
  • 01:45Isolation of Donor Cells
  • 02:14Oocyte Enucleation
  • 04:48Nuclear Transfer
  • 08:27Blastocyst Injection for Indirect 2 Step Cloning
  • 10:07Embryo Transfer
  • 11:56Representative Results
  • 12:59Conclusion

Summary

Traduzione automatica

Traduzione automatica

We demonstrate here that epigenetic reprogramming via Somatic Cell Nuclear Transfer (SCNT) can be used as a tool to generate mouse models with pre-defined T cell receptor (TCR) specificities. These transnuclear mice express the corresponding TCR from their endogenous locus under the control of the endogenous promoter.

Tags

Transnuclear MicePre-defined T Cell Receptor SpecificitiesToxoplasma GondiiSCNTLymphocytesGenetic RecombinationAntigen-specific T Cell ReceptorT Cell DevelopmentT Cell FunctionLong-term CultureAntigen StimulationHigh AffinityRandom Genomic IntegrationExpression LevelKineticsEpigenetic ReprogrammingSomatic Cell Nuclear TransferEmbryonic Stem CellsMouse ModelsEndogenous LociDisease Models

Article

Embed

AGGIUNGI ALLA PLAYLIST

Usage Statistics

Video correlati

check_url/it/2168?article_type=v

Obtaining Highly Purified Toxoplasma gondii Oocysts by a Discontinuous Cesium Chloride Gradient
check_url/it/2168?article_type=v

Toxoplasma gondii Cyst Wall Formation in Activated Bone Marrow-derived Macrophages and Bradyzoite Conditions
check_url/it/2168?article_type=v

Derivation of Thymic Lymphoma T-cell Lines from Atm-/- and p53-/- Mice
check_url/it/2168?article_type=v

A Genetic Screen to Isolate Toxoplasma gondii Host-cell Egress Mutants
check_url/it/2168?article_type=v

Genetic Manipulation in Δku80 Strains for Functional Genomic Analysis of Toxoplasma gondii
check_url/it/2168?article_type=v

Forward Genetics Screens Using Macrophages to Identify Toxoplasma gondii Genes Important for Resistance to IFN-γ-Dependent Cell Autonomous Immunity
check_url/it/2168?article_type=v

Retroviral Transduction of Bone Marrow Progenitor Cells to Generate T-cell Receptor Retrogenic Mice
check_url/it/2168?article_type=v

Development of Stem Cell-derived Antigen-specific Regulatory T Cells Against Autoimmunity
check_url/it/2168?article_type=v

Identification of Mediators of T-cell Receptor Signaling via the Screening of Chemical Inhibitor Libraries
check_url/it/2168?article_type=v

Single-cell Screening Method for the Selection and Recovery of Antibodies with Desired Specificities from Enriched Human Memory B Cell Populations

Read Article