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Fabrication of Micro-tissues using Modules of Collagen Gel Containing Cells
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JoVE Journal Bioingegneria
Fabrication of Micro-tissues using Modules of Collagen Gel Containing Cells

Fabrication of Micro-tissues using Modules of Collagen Gel Containing Cells

DOI:
10.3791/2177-v

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09:28 min

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December 13, 2010

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, , , , , , , , , ,
1Institute of Biomaterials and Biomedical Engineering / Department of Chemical Engineering and Applied Chemistry,University of Toronto, 2Institute of Biomaterials and Biomedical Engineering,University of Toronto

Capitoli

  • 01:16Neutralization of Collagen
  • 02:36Gelling of Collagen in Tubing
  • 03:31Cutting Tubing and Removing Collagen Modules
  • 04:57Coating Modules with Endothelial Cells
  • 06:46Results of Fabricating Micro-tissues Using Cylindrical Collagen Gels
  • 09:04Conclusion

Summary

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Creation of micro-tissues using cylindrical collagen gels, called modules, that contain embedded cells and which surface is coated with endothelial cells.

Tags

FabricationModulesCollagen GelCellsUniformScalableGelable MaterialsCrosslinkable MaterialsSize ShrinkageEndothelial CellsDiffusion LimitOxygenNutrientsModular ConstructionCell TypesNeutralizing CollagenEmbedding CellsGelling CollagenCutting ModulesCoating Endothelial Cells

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