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埃及斑蚊胚胎显微注射获得转基因蚊子
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Biologia
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JoVE Journal Biologia
Microinjection of A. aegypti Embryos to Obtain Transgenic Mosquitoes
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埃及斑蚊胚胎显微注射获得转基因蚊子

DOI:
10.3791/219-v

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17:39 min

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July 04, 2007

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, ,
1Department of Molecular Biology and Biochemistry,University of California, Irvine (UCI), 2Department of Molecular Biology and Biochemistry, Department of Microbiology and Molecular Genetics,University of California, Irvine (UCI)

Capitoli

  • 00:00Introduction
  • 00:29Embryo Collection
  • 02:26Preparing Microinjection Needles
  • 05:30Alignment and Manipulation of Embryos
  • 08:22Dessication of Embryos
  • 10:18Micromanipulation and Microinjection
  • 13:59Post Injection Treatment of Embryos
  • 15:25Hatching of Larvae
  • 16:50Conclusion

Summary

Traduzione automatica

Traduzione automatica

在这个视频，Nijole Jasinskiene演示方法生成的转基因埃及伊蚊蚊子，登革热的病媒。正确准备显微注射针，dessicating胚胎，并执行显微注射​​技术演示。

Tags

MicroinjectionA. Aegypti EmbryosTransgenic MosquitoesDengue FeverNijole JasinskieneMethodologyVectorsTechniquesMicroinjection NeedlesDessicating Embryos

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