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Strategies for Study of Neuroprotection from Cold-preconditioning
JoVE Journal
Neuroscienze
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JoVE Journal Neuroscienze
Strategies for Study of Neuroprotection from Cold-preconditioning

Strategies for Study of Neuroprotection from Cold-preconditioning

DOI:
10.3791/2192-v

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16:27 min

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September 02, 2010

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, ,
1Department of Neurology,The University of Chicago Medical Center

Capitoli

  • 00:00Titolo
  • 00:50Introduction
  • 01:46Preparation and Maintenance of Hippocampal Slice Cultures
  • 05:34Pre-screen Vitality of Slice Cultures
  • 06:47Cold-preconditioning
  • 07:36Excitotoxic Injury
  • 10:43Representative Imaging Results
  • 14:24Discussion
  • 15:36Conclusion

Summary

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We seek to define the neural immune signaling responsible for cold-preconditioning as means to identify novel targets for therapeutics development to protect brain before injury onset. We present strategies for such work that require biological systems, experimental manipulations plus technical capacities that are highly reproducible and sensitive.

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NeuroprotectionCold-preconditioningNeurological InjuryAnesthesiaSurgical ProceduresPreconditioning TreatmentsNeural Immune SignalingTherapeutics DevelopmentPro-inflammatory Mediator SignalingPhysiological Conditioning HormesisIrritative StimuliAdaptationImmune SignalingHippocampal Slice CulturesIn Vitro ModelMulti-synaptic Neural NetworksMacrogliaMicroglia

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