È necessario avere un abbonamento a JoVE per visualizzare questo. Accedi o inizia la tua prova gratuita.
Capitoli
- 00:05Titolo
- 01:04Introduction
- 01:17Thawing and Pre-Activation of CD34+ Cells
- 03:25Retroviral Transduction by Virus Pre-Loading
- 05:04Cell Sorting and Collection of Transduced Cells
- 06:08CFC Assay
- 08:14Giemsa Staining
- 10:29CFC Assay Results with Geimsa Stain Analyses
- 11:07Conclusion
The colony forming cell (CFC) assay is an in vitro assay in which hematopoietic progenitors form colonies in a semi-solid medium. A combination of colony morphology, cell morphology, and flow cytometry are used to assess the ability of the progenitors to proliferate and differentiate along the different hematopoietic lineages.
Tags
Colony Forming Cell (CFC) AssayHuman Hematopoietic CellsBone MarrowCord BloodPeripheral BloodHematopoiesisLeukemogenesisLymphoid LineageMyeloid LineageSemisolid MediumProliferationDifferentiation PatternColoniesInput CellsFlow CytometryGiemsa-stained SlidesMyeloid DifferentiationOncogenesPrimary Human CD34+ CellsRetroviral ConstructNUP98-HOXA9MSCV-IRES-GFP
