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1Department of Basic Science and Craniofacial Biology,College of Dentistry, New York University, 2Department of Internal Medicine and Department of Psychiatry,School of Medicine, Washington University in St. Louis, 3Veterans Affairs Medical Center, 4School of Dental Medicine, Department of Biochemistry,University of Pennsylvania-School of Medicine, 5Monell Chemical Senses Center, 6Monell Chemical Senses Center
Capitoli
- 00:00Titolo
- 00:11Introduction
- 01:54Subject Preparation
- 03:39Tongue Papillae Removal Technique
- 06:54Representative Papillae Tissue Results
- 08:55Conclusion
Knowledge of molecular mechanisms underlying gustatory transduction has recently enjoyed significant advances, largely due to using animal models. However, the wide diversity in taste sensitivity and specificity among mammals warrants studies in human tissue. We describe a biopsy technique to collect living taste cells from the papillae on human tongue.
Tags
TechniqueCollectFungiform PapillaeHuman TongueSense Of TasteCriticalQuality Of FoodPotentially IngestedStimulate Metabolic ProcessesAlimentary CanalDigestionProgressUnderstandingBiochemical EventsMolecular EventsTaste TransductionAnimal ModelsReceptor SpecificityReceptor DensityBiopsy TechniqueRounded Pink Anterior StructuresDiameterTaste BudsIsolation Of Viable Taste Bud CellsIn Situ HybridizationImmunohistochemistryMolecular Biology TechniquesIdentification Of Taste-specific Novel Proteins
