È necessario avere un abbonamento a JoVE per visualizzare questo. Accedi o inizia la tua prova gratuita.
1Department of Basic Science and Craniofacial Biology,College of Dentistry, New York University, 2Department of Internal Medicine and Department of Psychiatry,School of Medicine, Washington University in St. Louis, 3Veterans Affairs Medical Center, 4School of Dental Medicine, Department of Biochemistry,University of Pennsylvania-School of Medicine, 5Monell Chemical Senses Center, 6Monell Chemical Senses Center
Capitoli
- 00:00Titolo
- 00:11Introduction
- 01:54Subject Preparation
- 03:39Tongue Papillae Removal Technique
- 06:54Representative Papillae Tissue Results
- 08:55Conclusion
Die Kenntnis der zugrunde liegenden molekularen Mechanismen Geschmacks Transduktion hat kürzlich bedeutende Fortschritte genossen, vor allem aufgrund der Verwendung von Tiermodellen. Doch die große Vielfalt in Geschmack Sensitivität und Spezifität bei Säugetieren Optionsscheinen Studien in menschlichem Gewebe. Wir beschreiben eine Biopsie-Technik auf lebende Geschmack Zellen aus der Papillen auf die menschliche Zunge zu sammeln.
Tags
TechniqueCollectFungiform PapillaeHuman TongueSense Of TasteCriticalQuality Of FoodPotentially IngestedStimulate Metabolic ProcessesAlimentary CanalDigestionProgressUnderstandingBiochemical EventsMolecular EventsTaste TransductionAnimal ModelsReceptor SpecificityReceptor DensityBiopsy TechniqueRounded Pink Anterior StructuresDiameterTaste BudsIsolation Of Viable Taste Bud CellsIn Situ HybridizationImmunohistochemistryMolecular Biology TechniquesIdentification Of Taste-specific Novel Proteins
