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A Cell-to-cell Macromolecular Transport Assay in Planta Utilizing Biolistic Bombardment
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JoVE Journal Biologia
A Cell-to-cell Macromolecular Transport Assay in Planta Utilizing Biolistic Bombardment

A Cell-to-cell Macromolecular Transport Assay in Planta Utilizing Biolistic Bombardment

DOI:
10.3791/2208-v

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07:14 min

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August 27, 2010

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, , ,
1Department of Biochemistry and Cell Biology,State University of New York at Stony Brook, 2Bio-Medical Engineering Department,NED University of Engineering and Technology

Capitoli

  • 00:00Titolo
  • 00:51Introduction
  • 01:24Target Plant Growth
  • 02:32Biolistic Delivery of DNA-coasted Microparticles
  • 04:09Imaging of Protein Expression
  • 05:17Representative Results for a Cell-to-cell Macromelucular Transport Assay
  • 06:38Conclusion

Summary

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Macromolecular trafficking between plant cells can be assessed by transiently expressing a fluorescently-tagged protein of interest and analyzing its intra- and intercellular distribution by confocal microscopy.

Tags

Cell-to-cell Macromolecular TransportAssayBiolistic BombardmentFluorescently Tagged ProteinEncoding DNA ConstructConfocal MicroscopySymplastic Protein TransportArabidopsis ThalianaNicotiana BenthamianaN. Tabacum (tobacco)

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