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Profiling of Pre-micro RNAs and microRNAs using Quantitative Real-time PCR (qPCR) Arrays
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Biologia
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JoVE Journal Biologia
Profiling of Pre-micro RNAs and microRNAs using Quantitative Real-time PCR (qPCR) Arrays

Profiling of Pre-micro RNAs and microRNAs using Quantitative Real-time PCR (qPCR) Arrays

DOI:
10.3791/2210-v

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10:58 min

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December 03, 2010

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, ,
1Department of Microbiology and Immunology, Lineberger Comprehensive Cancer Center, Center for AIDS Research,University of North Carolina at Chapel Hill

Capitoli

  • 00:05Titolo
  • 01:21Introduction
  • 02:00qPCR Reaction Sample Setup
  • 03:03Setup of the Pre-miRNA Assay Using the Freedom Tecan Evo Robot
  • 05:17Setup of the Pre-miRNA Assay Using the Matrix Electronic Multichannel Pipette
  • 08:00Representative Results for Profiling of Pre-micro RNAs and MicroRNAs using qPCR Arrays

Summary

Traduzione automatica

Traduzione automatica

We will demonstrate the setup and analysis of pre-microRNA 96-well arrays for QPCR using a robot as well as by hand with a Thermo Scientific Matrix multichannel pipette.

Tags

ProfilingPre-micro RNAsMicroRNAsQuantitative Real-time PCRQPCR ArraysGene ExpressionDetection LimitInput AmountsAutomated QPCR SetupReproducibilityHigh-throughput ExperimentsProfiling Of GenesInternal Plate ControlsExperimental VariablesPrimer PairsSmall Non-coding RNAsPost-transcriptional Level RegulationPrimary MiRNA TranscriptPrecursor MiRNAMature MiRNA Levels

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