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Enzyme-linked Immunospot Assay (ELISPOT): Quantification of Th-1 Cellular Immune Responses Against Microbial Antigens
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Immunologia e infezioni
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JoVE Journal Immunologia e infezioni
Enzyme-linked Immunospot Assay (ELISPOT): Quantification of Th-1 Cellular Immune Responses Against Microbial Antigens

Enzyme-linked Immunospot Assay (ELISPOT): Quantification of Th-1 Cellular Immune Responses Against Microbial Antigens

DOI:
10.3791/2221-v

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06:13 min

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November 23, 2010

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, , ,
1Department of Medicine,Vanderbilt University School of Medicine, 2Department of Microbiology and Immunology,Vanderbilt University School of Medicine

Capitoli

  • 00:05Titolo
  • 00:58Coating the ELISPOT Plate
  • 01:45Cell Preparation
  • 02:08Setting up the Plate
  • 03:25Developing the ELISPOT Plate
  • 05:53Conclusion

Summary

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Identification of microbial targets of adaptive immunity in idiopathic diseases can be accomplished by the use of the enzyme-linked immunospot assay.

Tags

Enzyme-linked Immunospot AssayELISPOTCellular Immune ResponsesMicrobial AntigensTh-1 Immune ResponseDiagnostic ToolImmunosuppressed PopulationsHIV InfectionTransplantationSteroid UseTh1 Or Th2 Immune ResponsePeripheral BloodBronchoalveolar LavageCerebral Spinal FluidAscitesEpitopes Within Microbial ProteinsImmune Responses Over TimeDual Color ELISPOT AssaysCytokinesExtrapulmonary TuberculosisInfectious AntigensAutoimmunity

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