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微机械动态细胞培养平台

JoVE Journal
Bioingegneria

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JoVE Journal Bioingegneria

Microfabricated Platforms for Mechanically Dynamic Cell Culture

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微机械动态细胞培养平台

DOI:

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15:21 min

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December 26, 2010

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Christopher Moraes², Yu Sun², Craig A. Simmons^2,3

¹Department of Mechanical and Industrial Engineering,University of Toronto, ²Institute of Biomaterials and Biomedical Engineering,University of Toronto, ³Faculty of Dentistry,University of Toronto

Capitoli

00:05Titolo
01:05Introduction
01:31Sandwich Mold Fabrication
03:27Constructing the Multilayer Device
06:45Microactuator Modification for Active 2D Substrates
10:01Device Modifications for Active 3D Hydrogels
12:11In Situ Polymerization
13:51Representative Results for Device Fabrication, Characterization, and Operation
14:57Conclusion

Summary

Traduzione automatica

在这个协议中，我们展示的微驱动器阵列的垂直流离失所该技术是基于职位的制造，以及如何可以修改此基础技术，进行二维和三维培养高吞吐量的机械动态细胞培养范例。

Tags

Microfabricated Platforms Mechanically Dynamic Cell Culture Mechanobiological Stimuli Drug Discovery Cell Biology Studies Bioreactor Technologies Mechanical Stimuli High-throughput Format Microactuator Array Vertically Displaced Posts Two-dimensional Culture Paradigms Three-dimensional Culture Paradigms

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