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维持细胞培养基的Wolbachia
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Biologia
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JoVE Journal Biologia
Maintaining Wolbachia in Cell-free Medium
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维持细胞培养基的Wolbachia

DOI:
10.3791/223-v

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13:33 min

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July 04, 2007

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,
1Malaria Research Institute, Bloomberg School of Public Health,Johns Hopkins University

Capitoli

  • 00:06Introduction
  • 01:24Lysing Infected Anopheles Cells
  • 03:34Filtering Lysates and Isolation of Wolbacchia
  • 09:31Assay of Wolbacchia Viability Using Live/Dead Stain
  • 12:11Conclusion
  • 13:22End Credits

Summary

Traduzione automatica

Traduzione automatica

本视频介绍了净化按蚊细胞系的Wolbachia pipientis，然后在无细胞培养基培养的共生细菌的方法。细菌的生存能力的检测证明。

Tags

WolbachiaCell-free MediumVideo ProtocolPurifySymbiontsCultured Mosquito CellsFluorescent AssayViabilityExtracellular PhaseReplicateEukaryotic CellsMicro-injectionDNA Extraction

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