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Maintaining Wolbachia in Cell-free Medium
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JoVE Journal Biologia
Maintaining Wolbachia in Cell-free Medium

Maintaining Wolbachia in Cell-free Medium

DOI:
10.3791/223-v

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13:33 min

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July 04, 2007

•

,
1Malaria Research Institute, Bloomberg School of Public Health,Johns Hopkins University

Capitoli

  • 00:06Introduction
  • 01:24Lysing Infected Anopheles Cells
  • 03:34Filtering Lysates and Isolation of Wolbacchia
  • 09:31Assay of Wolbacchia Viability Using Live/Dead Stain
  • 12:11Conclusion
  • 13:22End Credits

Summary

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This video describes a method for purifying Wolbachia pipientis from an Anopheles gambiae cell line and then culturing the endosymbiont in cell-free medium. An assay for viability of the bacterium is demonstrated.

Tags

WolbachiaCell-free MediumVideo ProtocolPurifySymbiontsCultured Mosquito CellsFluorescent AssayViabilityExtracellular PhaseReplicateEukaryotic CellsMicro-injectionDNA Extraction

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