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Cryopreserving and Recovering of Human iPS Cells using Complete KnockOut Serum Replacement Feeder-Free Medium
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Biologia
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JoVE Journal Biologia
Cryopreserving and Recovering of Human iPS Cells using Complete KnockOut Serum Replacement Feeder-Free Medium

Cryopreserving and Recovering of Human iPS Cells using Complete KnockOut Serum Replacement Feeder-Free Medium

DOI:
10.3791/2237-v

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06:47 min

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July 15, 2010

•

,
1GIBCO,Life Technologies

Capitoli

  • 00:04Titolo
  • 00:44Introduction
  • 01:31Cryopreserving iPSCs
  • 03:48Thawing iPSCs
  • 05:54Representative Results
  • 06:24Conclusion

Summary

Traduzione automatica

Traduzione automatica

This protocol describes the detailed procedure for cryopreserving human iPS cells in KnockOut SR cryopreservation medium and recovering these cells in complete KnockOut SR Feeder Free (KSR-FF) medium or feeder-based KnockOut SR medium.

Tags

CryopreservingRecoveringHuman IPS CellsComplete KnockOut Serum ReplacementFeeder-free MediumReprogrammingFibroblastsPluripotent CellsDrug DiscoveryCell TherapyBasic ResearchGIBCO Media And ReagentsKnockout DMEMKnockout Serum ReplacementEmbryonic Stem Cell GrowthIPS Cell CultureFeeder-free CultureKnockout SR Growth Factor CocktailTraditional Culture MethodsMouse Or Human Fibroblast Feeder LayersFeeder-conditioned MediumLabor-intensiveHard To ScaleUndifferentiated Cells

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