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Optic Nerve Transection: A Model of Adult Neuron Apoptosis in the Central Nervous System
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JoVE Journal Neuroscienze
Optic Nerve Transection: A Model of Adult Neuron Apoptosis in the Central Nervous System

Optic Nerve Transection: A Model of Adult Neuron Apoptosis in the Central Nervous System

DOI:
10.3791/2241-v

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12:06 min

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May 12, 2011

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, ,
1Department of Surgery,University of Toronto

Capitoli

  • 00:05Titolo
  • 01:22Surgical Approach
  • 05:59Accessing the Optic Nerve
  • 08:31Closing and Recovery
  • 09:35Representative Results
  • 11:27Conclusion

Summary

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Optic Nerve transection is a widely used model of adult CNS injury. Ninety percent of retinal ganglion cells (RGCs) whose axons are completely transected (axotomy) die within 14 days after axotomy. This model is easily amenable to experimental manipulations and highly reproducible.

Tags

Optic Nerve TransectionAdult Neuron ApoptosisCentral Nervous SystemRetinal Ganglion CellsOptic NerveAxotomizedApoptotic Neuronal Cell DeathCNS ModelVitreous ChamberDrug DeliveryIntraocular InjectionsShort Interfering RNAs (siRNAs)PlasmidsViral VectorsSuperior ColliculusApoptotic MechanismsNeuronal PopulationCell Survival QuantificationFluorescent Tracer

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