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Large Scale Zebrafish-Based In vivo Small Molecule Screen
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Biologia
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JoVE Journal Biologia
Large Scale Zebrafish-Based In vivo Small Molecule Screen

Large Scale Zebrafish-Based In vivo Small Molecule Screen

DOI:
10.3791/2243-v

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07:03 min

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December 30, 2010

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, , ,
1Division of Cardiovascular Medicine, Department of Medicine,Vanderbilt University School of Medicine, 2Department of Pharmacology,Vanderbilt University School of Medicine, 3Vanderbilt Institute of Chemical Biology,Vanderbilt University School of Medicine, 4Research Medicine, Veterans Affairs TVHS,Vanderbilt University School of Medicine

Capitoli

  • 00:05Titolo
  • 00:40Introduction
  • 01:02Zebrafish Egg Collection and 96-Well Plate Preparatin
  • 02:52Small Molecule Library
  • 04:23Screening for Effects of Small Molecules by Visual Inspection of Phenotypes
  • 05:51Small Molecule Detection with Zebrafish Chemical Screening Assay
  • 06:21Conclusion

Summary

Traduzione automatica

Traduzione automatica

Zebrafish has emerged as a powerful in vivo platform for phenotype-based drug screens and chemical genetic analysis. Here, we demonstrate a simple, practical method for large-scale screening of small molecules using zebrafish embryos.

Tags

ZebrafishIn VivoSmall Molecule ScreenPhenotype-based Drug ScreensChemical Genetic AnalysisLarge-scale ScreeningZebrafish Embryos

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