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在完整的果蝇幼虫体内的亚细胞分辨率成像
JoVE Journal
Biologia
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JoVE Journal Biologia
In vivo Imaging of Intact Drosophila Larvae at Sub-cellular Resolution
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在完整的果蝇幼虫体内的亚细胞分辨率成像

DOI:
10.3791/2249-v

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17:51 min

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September 10, 2010

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, , , , ,
1Junior Research Group Synaptic Plasticity, Hertie Institute for Clinical Brain Research,University of Tübingen, 2Graduate School of Cellular and Molecular Neuroscience,University of Tübingen

Capitoli

  • 00:00Titolo
  • 00:05Introduction
  • 02:01Preparing Larva
  • 03:00Assembly of the Imaging Chamber
  • 04:36Anesthetization of the Larva
  • 07:48Imaging
  • 14:39Recovery from Anesthetization

Summary

Traduzione automatica

Traduzione automatica

本协议描述了一种可靠的方法，麻醉和完整的成像<em>果蝇</em>幼虫。我们利用挥发性麻醉药氟烷允许重复成像，在亚细胞的分辨率和结构的重新鉴定几天<sup> 1</sup>。

Tags

In Vivo ImagingDrosophila LarvaeSub-cellular ResolutionOptical ImagingGenetically Encoded FluorophoresGenetic ToolsTransgenic Animal LinesAnesthetize LarvaeDesfluraneSynaptic PopulationsHigh Degree Of AnesthetizationSurvival RateTime-pointsProteins DynamicsGlutamate Receptor Subunit GluR-IIAEndogenous PromoterExon Trap Line FasII-GFP

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