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Capitoli
- 00:05Titolo
- 02:23Performing the Capture Assay and Controls
- 08:26Tryptic Digest of Captured Proteins and Preparation of Peptides for LC-MS/MS
- 09:50Peptide and Protein Identification via Automated Sequence Database Search
- 12:33Representative Results of CCMS Experiments
- 16:00Conclusion
Capture Verbindingen zijn trifunctionele kleine moleculen om de complexiteit van het proteoom te verminderen door functionele omkeerbare klein molecuul-eiwit interacties, gevolgd door foto-crosslinking en zuivering. Hier gebruiken we een Capture Compound met<em> S</em>-Adenosyl-<sub> L</sub>-Homocysteine-bindende als selectiviteit functie om methyl-isoleren van een<em> Escherichia coli</em> Hele cellysaat en identificeren hen door MS.
Tags
MethyltransferasesS-adenosyl-L-homocysteine-binding ProteinsCapture Compound Mass SpectrometryAffinity ChromatographyActivity-based Protein ProfilingTrifunctional Capture CompoundsPhoto-crosslinkingSmall Molecule-protein InteractionsSelectivity FunctionSAHSorting FunctionBiotinSolid PhaseStreptavidin Magnetic BeadsOff-bead ConfigurationOn-bead ConfigurationSmall Molecule Of InterestSAMSAM-dependent MethyltransferasesDNA Methylation
