È necessario avere un abbonamento a JoVE per visualizzare questo. Accedi o inizia la tua prova gratuita.
Capitoli
- 00:00Titolo
- 01:30Hanging Drop
- 06:21Sitting Drop
- 07:05Microbatch Procedure
- 07:51Representative Results for Protein Crystallization
- 08:50Conclusion
The 3-D structure of a molecule provides a unique understanding of how the molecule functions. The principal method for structure determination at near-atomic resolution is X-ray crystallography. Here, we demonstrate the current methods for obtaining three-dimensional crystals of any given macromolecule that are suitable for structure determination by X-ray crystallography.
Tags
Protein CrystallizationX-ray CrystallographyBiological MacromoleculesAtomic Resolution StructuresProtein Data Bank (rcsb-PDB)Macromolecular StructuresCrystallographic StudiesPurificationHomogeneityHigh Resolution CrystalsSupersaturationConcentrationAggregationPrecipitationNucleationProtein CrystalsVapor DiffusionBatch Crystallization
