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Immunologia e infezioni

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胶粘剂基于磁带的采样和荧光组合原位杂交快速检测沙门氏菌新鲜农产品

JoVE Journal
Immunologia e infezioni

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JoVE Journal Immunologia e infezioni

Combination of Adhesive-tape-based Sampling and Fluorescence in situ Hybridization for Rapid Detection of Salmonella on Fresh Produce

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胶粘剂基于磁带的采样和荧光组合原位杂交快速检测沙门氏菌新鲜农产品

DOI:

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09:10 min

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October 18, 2010

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Bledar Bisha, Byron F. Brehm-Stecher

¹Center for Meat Safety and Quality, Department of Animal Sciences,Colorado State University, ²Rapid Microbial Detection and Control Laboratory, Department of Food Science and Human Nutrition,Iowa State University

Capitoli

00:00Titolo
01:32Introduction
02:07Surface Sampling with Sterile Adhesive Tape
03:33Solid-phase Enrichment
04:54Fixation and Dehydration
05:35Hybridization
06:46Detection
07:43FISH and FACS Analyses on Tomatoes
08:32Conclusion

Summary

Traduzione automatica

本协议描述了简单的基于磁带的粘合剂为番茄等新鲜农产品表面取样的方法，全细胞快速检测<em>沙门氏菌</em>用荧光<em>原位</em>杂交（FISH）。

Tags

Adhesive-tape-based Sampling Fluorescence In Situ Hybridization Rapid Detection Salmonella Fresh Produce Culture-independent Detection Cell-charged Tapes Solid-phase Enrichment Liquid Surface Miniculture DNA Probe Cocktail Hybridization Fluorescence Microscopy

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