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Optimized Transfection Strategy for Expression and Electrophysiological Recording of Recombinant Voltage-Gated Ion Channels in HEK-293T Cells
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Biologia
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JoVE Journal Biologia
Optimized Transfection Strategy for Expression and Electrophysiological Recording of Recombinant Voltage-Gated Ion Channels in HEK-293T Cells

Optimized Transfection Strategy for Expression and Electrophysiological Recording of Recombinant Voltage-Gated Ion Channels in HEK-293T Cells

DOI:
10.3791/2314-v

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12:59 min

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January 19, 2011

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, ,
1Department of Biology,University of Waterloo

Capitoli

  • 00:00Titolo
  • 02:36Transfection of HEK-293T with Recombinant Ion Channel cDNAs
  • 04:52Plating Transfected HEK-293T Cells in Preparation for Electrophysiological Recording
  • 07:36Electrophysiological Recording of Recombinant Channels in HEK-293T Cells
  • 11:29Representative Imaging and Patch Clamp Recordings
  • 12:13Conclusion

Summary

Traduzione automatica

Traduzione automatica

Reliable method for highly efficient in vitro expression and subsequent electrophysiological recording of recombinant voltage-gated ion channels in cultured human embryonic kidney cells (HEK-293T).

Tags

OptimizedTransfection StrategyExpressionElectrophysiological RecordingRecombinant Voltage-gated Ion ChannelsHEK-293T CellsIn VitroCultured Human Embryonic Kidney CellsGlass CoverslipsDensityElectrophysiological RecordingContattaConfounding EffectsPlasma MembraneWhole-cell Patch Clamp RecordingDetectable CurrentsNoise LevelsIncubation PeriodsMembrane ExpressionCell-coverslip AdhesionMembrane StabilityTemperatureTransfected CellsHigh ConfluencyIon Channel Protein ExpressionGlass CoverslipsIncubation PeriodCell Attachment

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