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Membrane Potentials, Synaptic Responses, Neuronal Circuitry, Neuromodulation and Muscle Histology Using the Crayfish: Student Laboratory Exercises
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Capitoli
- 00:00Titolo
- 01:30Surgical Procedure to Expose the Abdominal Extensor Muscles
- 03:35Intracellular Recording from the Abdominal Extensors Muscle Fibers
- 05:50Results: Effect of K+ Concentration on Intracellular Recordings of Extensor Muscle Fibers
- 06:42Neuroanatomical Observations of Abdominal Extensor Muscles
- 08:03Recording Synaptic Responses in Abdominal Extensor Muscles
- 10:00Results: Comparing Synaptic Responses of DEL and SEL Muscles
- 11:19Intracellular Recording of EPSPs and IPSPs from Superficial Flexor Muscles
- 12:35Results: EPSPs of Superficial Flexor Muscle Fibers
- 12:46Extracellular Recording from Motor Neurons of the Superficial
- 14:27Results: Motor Neuron Response to Sensory Stimulation and Serotonin
- 15:30Conclusion
The experiments demonstrate an easy approach for students to gain experience in examining muscle structure, synaptic responses, the effects of ion gradients and permeability on membrane potentials. Also, a sensory-CNS-motor-muscle circuit is presented to show a means to test effects of compounds on a neuronal circuit.
Tags
Membrane PotentialsSynaptic ResponsesNeuronal CircuitryNeuromodulationMuscle HistologyCrayfishIon GradientsBiological MembraneIon ConcentrationK+PermeabilityAbdominal Extensor MusclesTonicPhasicMotor NeuronsSynaptic TransmissionSensory-CNS-motor Neuron-muscle CircuitCuticular Sensory StimulationNeuromodulatorsExperimental PreparationsPhysiological ApplicationsModel Invertebrate Preparations
