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印记基因的DNA甲基化的测定拟南芥胚乳

JoVE Journal
Biologia

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JoVE Journal Biologia

Determination of DNA Methylation of Imprinted Genes in Arabidopsis Endosperm

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印记基因的DNA甲基化的测定拟南芥胚乳

DOI:

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09:23 min

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January 28, 2011

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Matthew Rea, Ming Chen, Shan Luan, Drutdaman Bhangu, Max Braud, Wenyan Xiao

¹Department of Biology,Saint Louis University

Capitoli

00:05Titolo
01:11Genetic Crossing
02:52Isolation of Endosperm Tissues in Arabidopsis
03:46Bisulfite Treatment
06:20PCR Amplification
07:47Sequence Analysis and Determination of DNA Methylation Status: Representative Results
09:00Conclusion

Summary

Traduzione automatica

印记是在植物和哺乳动物繁殖的现象。 DNA甲基化起着重要作用的印迹机制。隔离胚乳和确定的印记基因的甲基化状态<em>拟南芥</em>可以是困难的。在这个协议中，我们介绍了如何隔离胚乳，并确定由亚硫酸氢钠测序的甲基化。

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DNA Methylation Imprinted Genes Arabidopsis Endosperm Epigenetic Mechanisms DNA Methyltransferases Loss Of DNA Methylation Imprinting Maternal And Paternal Alleles Reproduction Development Flowering Plants Double Fertilization Event MEDEA Gene FWA Gene DNA Methylation And Demethylation Imprinting Status Methylation Pattern Isolate Endosperm Genetic Cross

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