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Analysis of Pluripotent Stem Cells by using Cryosections of Embryoid Bodies
 
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Analysis of Pluripotent Stem Cells by using Cryosections of Embryoid Bodies

Article DOI: 10.3791/2344-v • 06:55 min • December 8th, 2010
December 8th, 2010 •
*1, *1, *1, 1, 1
1Laboratório Nacional de Céulas-Tronco Embrionárias (LaNCE), Instituto de Ciências Biomédicas, Universidade Federal do Rio de Janeiro (UFRJ), Brazil
* These authors contributed equally

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Pluripotent stem cells growing in suspension differentiate into embryoid bodies (EBs). Here we demonstrate how to obtain high quality EB cryosections useful for studying cellular and molecular aspects of embryogenesis, while preserving their organization as aggregates.

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Pluripotent Stem Cells Cryosections Embryoid Bodies ES Cells Differentiation Aggregates Ectoderm Mesoderm Endoderm Immunofluorescence In Situ Hybridization Target Proteins MRNA Tissue Section Cryosection Technique Analytical Procedures Histology Staining Analysis Hematoxilin And Eosin DAPI Oct4 Nestin Embryogenesis Tri-dimensional Spherical Structure
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