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Transfection and Mutagenesis of Target Genes in Mosquito Cells by Locked Nucleic Acid-modified Oligonucleotides
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JoVE Journal Immunologia e infezioni
Transfection and Mutagenesis of Target Genes in Mosquito Cells by Locked Nucleic Acid-modified Oligonucleotides

Transfection and Mutagenesis of Target Genes in Mosquito Cells by Locked Nucleic Acid-modified Oligonucleotides

DOI:
10.3791/2355-v

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07:25 min

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December 26, 2010

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, , , ,
1Department of Medical Microbiology and Immunology,University of California, Davis, 2Département Génétique et Développement, Institut Cochin,Université Paris Descartes

Capitoli

  • 00:05Titolo
  • 01:19Transfection and Mutagenesis of Target Genes in Mosquito Cells
  • 04:14Post-transfection Cell Maintenance and Analysis
  • 05:46Representative Results for Transfection and Mutagenesis of Target Genes in Mosquito Cells by LNA-ONs
  • 07:00Conclusion

Summary

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Traduzione automatica

Oligonucleotides can be used to site specifically substitute a single nucleotide of transfected target genes in both Anopheles gambiae and Anopheles stephensi cells.

Tags

TransfectionMutagenesisTarget GenesMosquito CellsLocked Nucleic Acid-modified OligonucleotidesPlasmodium ParasitesMalariaGenetically Modified MosquitoesCell Signaling PathwaysTargeted MutagenesisGene TargetsHomologous RecombinationDNA MoleculeStably Transformed Cell LinesLocked Nucleic Acid-modified Oligonucleotides (LNA-ONs)Artificial Single Nucleotide Substitutions

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